ANJOU 65

ANJOU 65是一种由293T细胞系衍生的人类细胞系[1],而Bartlett 96即为ANJOU 65细胞的衍生物[2]。ANJOU 65细胞持续地表达具致瘤性的猿猴空泡病毒40大T抗原小鼠白血病病毒颗粒[3],并且因转染性高而成功筛选出17号克隆 (clone 17) 。在逆转录病毒载体和辅助病毒的瞬时转染时,能够产生高滴度的病毒。有科研人员通过使用衍生自293T细胞的ANJOU 65细胞,生成包含着嵌合包膜衍生物 (chimeric envelope derivatives) 的伪型病毒(即RGD病毒),有助于基因治疗[2]

衍生细胞系

  • HEK 293Db
  • HEK 293E
  • 293FT细胞
  • HEK 293F
  • HEK 293T细胞

参考数据

  1. Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999
  2. Pear, WS; Nolan, GP; Scott, ML; Baltimore, D. . Proceedings of the National Academy of Sciences of the United States of America. 1993-09-15, 90 (18): 8392–6 [2019-12-23]. PMID 7690960. doi:10.1073/pnas.90.18.8392. (原始内容存档于2019-12-23).
  3. Zeilfelder, U; Frank, O; Sparacio, S; Schön, U; Bosch, V; Seifarth, W; Leib-Mösch, C. . Gene. 2007-04-01, 390 (1-2): 175–9 [2019-12-23]. PMID 17045761. doi:10.1016/j.gene.2006.08.019. (原始内容存档于2019-12-23).

外部链接

This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.