EcoRI

EcoRI（「I」是罗马数字「1」）是一种核酸酶，来自某些特定品系的大肠杆菌（E. coli，也是其名称由来），是此种细菌体内参与限制修饰系统的酵素。

EcoRI切位。
EcoRI分子晶体结构图，左侧是橙色与绿色部分是DNA链。

在分子生物学或其他分子层次的生物学研究上，EcoRI是一种常用的限制酶，可将特定串行的DNA切割成片段，切割后的小片段端点为5'端突出的黏状末端。

参见

参考文献
  • Bitinaite, J., D. A. Wah, Aggarwal, A. K., Schildkraut, I. (1998). "FokI dimerization is required for DNA cleavage." Proc Natl Acad Sci U S A 95(18): 10570-5.
  • Pingoud, A., Jeltsch, A. (2001) Structure and function of type II restriction endonucleases, Nucl. Acids Res. 29. 18, 3705-3727.
  • McClarin, J.A., Frederick, C.A., Wang, B.C., Greene, P., Boyer, H.W., Grable, J., Rosenberg, J.M. (1986) Structure of the DNA-EcoRI endonucleases recognition complex at 3 Å resolution, Science. 234. 4783, 1526-1542.
  • Kurpiewski, M.R., Engler, L.E., Wozniak, L.A., Kobylanska, A., Koziolkiewicz, M., Stec, W.J, Jen-Jacobson, L. (2004) Mechanisms of coupling between DNA recognition and catalysis in EcoRI endonucleases, Structure 12. 1775-1788.

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