锁核酸
锁核酸(Locked nucleic acid,LNA)是一种经过修饰的RNA,LNA中一部分核糖上的2'与4'碳链接在一起。一般可见于A-DNA或RNA。
这类核酸可以增加引子或探针(probe)的融解温度(Tm值),加强这些实验用物质的稳定性,可应用在即时聚合酶连锁反应(real-time PCR)等技术中。
参考文献
- Kaur, H.; Arora, A.; Wengel, J.; Maiti, S. . Biochemistry. 2006, 45 (23): 7347–55. doi:10.1021/bi060307w.
外部链接
- A predictor for the physical properties of LNA modified oligonucleotides
- LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA (页面存档备份,存于)
- LNA: a versatile tool for therapeutics and genomics (页面存档备份,存于)
- Alternative Nucleic Acid Analogues for Programmable Assembly: Hybridization of LNA to PNA (页面存档备份,存于)
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